Histology of the Red Cells |Histology|


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Ok, once again – Histology. Tadaaa! =)
Ok, this is just a relax discussion, nothing is too complicated, we can get through in a blink of an eye lol
Let us start

Introduction

Erythrocytes or RBCs are :-

1) Biconcave in shape, makes it paler at the center
2) Lifespan of 120 days
3) Produced in red marrow; regulated by erythropoeitin
4) Consist of protein carrying O2 called haemoglobin
5) Stained by eosin dye, means it is basic in nature, with acidophilic in attraction
6) No nuclei and organelle, so that
+ it will give more space for heamoglobin
+ it wont use O2 that it carries (mitochondria uses O2)

Haemoglobin

1) Protein of tetrameric structure
2) Arrangement of haemoglobin
-Fetal heamoglobin (HBF)
+2 alpha
+2 gamma
-Adult haemoglobin
     + HBA1 (96%)
-2 alpha
-2 beta
+ HBA2 (2%)
-2 alpha
-2 gamma
+ HBF (2%)
3) Attached by iron containing heme
4) Globin
– Alpha globin
+ Chromosome 11
– Beta globin
+ Chromosome 16

Morphology of Erythrocyte
Red cells are biconcave in shape is due to its complex cytoskeleton arrangement. Let me try to simplify it
1) Vertical filament (Anchor)
+Ankyrin
+ Band 3 protein
+ Band 4 protein
2) Horizontal filament
+ Spectrin
3) Protein links both vertical and horizontal filaments
+ 15 actin monomeres

Formation

Erythrocyte formation is called as eryhtropoeisis. This process is then regulated by a hormone called erythropoeitin which released by peritubular capillary endothelial cells of the KIDNEY.

Erythropoeisis takes place at red marrow of long bone in children while flat bone in adults.

Below is the simplified diagram which might help you to understand the process

Pluripotent Hemopoeitic Stem Cell –> CFU-S –> BFU-E –> CFU-E –> Basophilic Erythroblast –> Polychromatophilic Erythroblast –> Orthochromatophilic/Eosinophilic Erythroblast –> ejection of nucleus –> Reticulocyte –> Erythrocyte

This whole process needs 15 days to complete one cycle
Meanwhile, reticulocyte needs 2 days to mature into erythrocyte. During this time, it eliminates its organelles.

Teminologies
1) CFU = Colony Forming Unit
2) BFU = Birth Forming Unit
3) CFU-E = Colony Forming Unit Erythrocyte
4) Stem cell = stem cell is not a comitted cell, means it can give rise to other types of cell. Such as:-
+ CFU-S
+ PHSC
5) Progenitor cell = this cell is a commited cell, means it will only give rise to a single mature cell. All CFU except CFU-S are progenitor cells
6) Precursor cell = immature cell, with ending of ‘blast’. Such as
+ Erythroblast

Thats all, thanks guys! =)

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2 responses to “Histology of the Red Cells |Histology|

    • Actually, its not less torture, I just try to make it less torturing haha. Medic is a tormenting torture, no one can deny that, just why not we try to take it as something that we enjoy learning with. Isn’t it? =) Goodluck to you Mirul =)

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